12/8/2023 0 Comments Replacement for 3hub![]() ![]() (Note that unless you have reliable Gigabit Ethernet service, you won’t be able to measure if the port is actually performing at full speed.) For each model in the test group, we verified the connection speed in Network Utility on a Mac, which displays the maximum link speed. Most hubs with Ethernet ports support gigabit speeds, meaning data can transfer at up to 1,000 megabits a second. Ethernet: A wired Ethernet connection is almost always faster than Wi-Fi and is more reliable, too. ![]() Most hubs support only a 30 Hz refresh rate (the image refreshes 30 times a second) at 4K resolution, but a handful now support a smoother 60 Hz rate, and we prefer those hubs that do. We connected each of the hubs via HDMI to a Philips 272P7VUBNB/27 monitor with the resolution set to 4K, and we used the Blur Busters UFO Motion Test to confirm the refresh rate. HDMI: A hub can be very useful at a desk, especially if you want to hook your computer up to an external monitor.Our measured speeds landed largely in the 400 to 425 MB/s range we eliminated any model that was noticeably slower. Most of the hubs we tested advertised USB 3.0 speeds, which max out at 640 megabytes per second on paper. Since SSDs are faster than spinning hard drives, our use of the T5 ensured that the speeds we measured weren’t hindered by the drive. We ran AJA System Test speed tests using the Samsung T5 Portable SSD (500 GB) to measure transfer speeds. USB-A speed: Although USB-A ports generally don’t support data-transfer rates as fast as those of USB-C or Thunderbolt 3 connectors, it’s still important to be able to transfer data quickly.Two USB-A 3.2 Gen 1, one USB-C 3.2 Gen 1, one USB-C power Two USB-A 3.2 Gen 2, one USB-C 3.2 Gen 2, one USB-C power Note that the Anker 10-Port 60W USB 3.0 Hub ships with only a USB-A–to–USB-B cable, so you’ll have to buy a separate USB-B–to–USB-C cable to connect it to many modern computers. As a bonus, it’s aesthetically inoffensive-it doesn’t look like it belongs with the greatest hits of ugly ’90s technology, unlike some of the other hubs we’ve tested. It has seven upward-facing USB-A 3.1 Gen 1 data ports with a helpful LED activity indicator for each it also has three 12-watt (2.4-amp) power ports just for charging. This approach may be a useful alternative to develop direct binding assays for kinases that do not adopt the DFG-out conformation while also avoiding the use of expensive kits, detection reagents, or radioactivity frequently employed with activity-based assays.Ĭhemical Genomics Centre of the Max Planck Society, Otto-Hahn-Strasse 15, D-44227 Dortmund, Germany.If you’re mainly concerned about connecting to your computer as many devices as you can, choose the Anker 10-Port 60W USB 3.0 Hub. Last, we demonstrate the power of this new FLiK labeling strategy to detect the binding of Type I ligands that induce conformational changes in the glycine-rich loop as a means of gaining affinity for the target kinase. We also detected the high-affinity binding of a clinically relevant and specific VEGFR2 inhibitor, and we provide structural details of its binding mode in p38alpha, in which it stabilizes the DFG-out conformation. Protein X-ray crystallography validated the assay results and identified a novel DFG-out binding mode for a quinazoline-based inhibitor in p38alpha kinase. Due to cross-talk between the glycine-rich loop and the activation loop in kinases, this alternative labeling approach can also detect ligands that stabilize inactive kinase conformations, including slow-binding Type II and Type III kinase inhibitors. Here we report the development of a new FLiK (Fluorescent Labels in Kinases) binding assay to detect small molecules that induce changes in the conformation of the glycine-rich loop. However, addressing enzymatically incompetent kinase conformations offers new opportunities for targeted therapies and is moving to the forefront of kinase inhibitor research. Traditionally, kinase inhibitors are identified in activity-based screening assays using enzymatically active kinase preparations to measure the perturbation of substrate phosphorylation, often resulting in the enrichment of classical ATP competitive (Type I) inhibitors. Targeting protein kinases with small organic molecules is a promising strategy to regulate unwanted kinase activity in both chemical biology and medicinal chemistry research. ![]()
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